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American Research Products recombinant stim-3 19-c356
Recombinant Stim 3 19 C356, supplied by American Research Products, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+stim+3/stim+3/pm28754800-117-46-50
Average 90 stars, based on 1 article reviews
recombinant stim-3 19-c356 - by Bioz Stars, 2026-09
90/100 stars

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Incubation:

Article Title: Frontline Science: Tim-3-mediated dysfunctional engulfment of apoptotic cells in SLE
Article Snippet: To confirm further the role of sTim-3 in plasma in Tim-3-mediated phagocytosis of apoptotic cells, sTim-3 in the plasma from SLE patients was depleted by incubating with the 4 mg anti-hTim-3 antibody (Catalog Number: MAB23651, Clone #344801; R&D Systems) at room temperature for 3 h, which had been conjugated to Protein A/G beads at 4°C overnight, and purified rat IgG1 was used as an isotype control. .. Then, pHrodo-SE-labeled apoptotic cells were incubated with sTim-3-depleted plasma from SLE patients for 60 min and cocultured with adherent-differentiated THP-1 cells (20:1) for 90 min. To explore further the effect of sTim-3 on Tim-3-mediated phagocytosis of apoptotic cells, pHrodo-SE-labeled apoptotic cells were incubated with 30 mg/ml recombinant sTim-3 (19-C356; ARP American Research Products, Waltham, MA, USA) for 60 min and cocultured with adherent-differentiated THP-1 cells for 90 min. ..

Clinical Proteomics:

Article Title: Frontline Science: Tim-3-mediated dysfunctional engulfment of apoptotic cells in SLE
Article Snippet: To confirm further the role of sTim-3 in plasma in Tim-3-mediated phagocytosis of apoptotic cells, sTim-3 in the plasma from SLE patients was depleted by incubating with the 4 mg anti-hTim-3 antibody (Catalog Number: MAB23651, Clone #344801; R&D Systems) at room temperature for 3 h, which had been conjugated to Protein A/G beads at 4°C overnight, and purified rat IgG1 was used as an isotype control. .. Then, pHrodo-SE-labeled apoptotic cells were incubated with sTim-3-depleted plasma from SLE patients for 60 min and cocultured with adherent-differentiated THP-1 cells (20:1) for 90 min. To explore further the effect of sTim-3 on Tim-3-mediated phagocytosis of apoptotic cells, pHrodo-SE-labeled apoptotic cells were incubated with 30 mg/ml recombinant sTim-3 (19-C356; ARP American Research Products, Waltham, MA, USA) for 60 min and cocultured with adherent-differentiated THP-1 cells for 90 min. ..

Recombinant:

Article Title: Frontline Science: Tim-3-mediated dysfunctional engulfment of apoptotic cells in SLE
Article Snippet: To confirm further the role of sTim-3 in plasma in Tim-3-mediated phagocytosis of apoptotic cells, sTim-3 in the plasma from SLE patients was depleted by incubating with the 4 mg anti-hTim-3 antibody (Catalog Number: MAB23651, Clone #344801; R&D Systems) at room temperature for 3 h, which had been conjugated to Protein A/G beads at 4°C overnight, and purified rat IgG1 was used as an isotype control. .. Then, pHrodo-SE-labeled apoptotic cells were incubated with sTim-3-depleted plasma from SLE patients for 60 min and cocultured with adherent-differentiated THP-1 cells (20:1) for 90 min. To explore further the effect of sTim-3 on Tim-3-mediated phagocytosis of apoptotic cells, pHrodo-SE-labeled apoptotic cells were incubated with 30 mg/ml recombinant sTim-3 (19-C356; ARP American Research Products, Waltham, MA, USA) for 60 min and cocultured with adherent-differentiated THP-1 cells for 90 min. ..



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